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ELISA (enzyme-linked immunosorbent assay)

Sensitive and specific analytical technique for determination of analytes present at very low concentrations in biological samples, in which either the tracer analyte or the antibody is bound to an enzyme; the product of the enzyme may be measured directly, or may be a catalyst or coenzyme for a second enzyme, giving considerable amplification, and hence permitting high sensitivity without the use of radioactive tracers.