Honey
Determination of moisture

  Determine the refractrometer reading of honey at 20C and calculate the percentage of moisture from the value given in the table if the determination is done below 20C, 0.00023 should be subtracted from the refractrometer reading for every degree decreasing temperature and same should be added for every degree of increasing temperature to the refractrometer reading. Using table

Example:-

1.482   at 32        = 1.482+ 0.00276

                  = 1.48476

                = 20.8%

• DETERMINATION OF TOTAL REDUSING SUGAR (TRS)

   Weigh out 2gm of honey in to a 500ml standard flask dilute with water and mix thoroughly condense and make the volume to 500ml with water. Using separate pipette, pipette out 5ml of each solution A and solution B in to a 250ml conical flask. Add about 16ml of honey solution from a burette add a few pumices and dilute with water heat to boiling over a flam and add 1ml methylene blue indicator solution while keeping the solution boiling complete the filtration with in 3 minutes the end point is indicated by the change the color from blue to red.

Calculation

Approximate TRS % by weight = 500*N*100
                                                              V*W

Where

            N = normality of Fehling’s solution.
            V = volume of honey solution required for titration.
           W =weight of honey taken.

Example:-

Weight of sample taken = 2.0355
    Titration value              = 17.7
          TRS                        =  500*0.0526096*100
                                                2.0355*17.7
                                          = 73.011